mouse: c57bl Search Results


c57 mouse embryonic fibroblasts mef  (ATCC)
91
ATCC c57 mouse embryonic fibroblasts mef
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57 Mouse Embryonic Fibroblasts Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mice  (Inotiv)
99
Inotiv mice
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
Mice, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c57bl 6 mouse immunoglobulin panel  (SouthernBiotech)
93
SouthernBiotech c57bl 6 mouse immunoglobulin panel
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57bl 6 Mouse Immunoglobulin Panel, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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wild type wt c57bl 6 mouse embryonic fibroblasts mef  (ATCC)
94
ATCC wild type wt c57bl 6 mouse embryonic fibroblasts mef
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
Wild Type Wt C57bl 6 Mouse Embryonic Fibroblasts Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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million j1 mes cells  (ATCC)
94
ATCC million j1 mes cells
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
Million J1 Mes Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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mouse sinusoidal endothelial cells  (AcceGen Biotechnology)
93
AcceGen Biotechnology mouse sinusoidal endothelial cells
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
Mouse Sinusoidal Endothelial Cells, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse%3A+c57bl/C57BL+6+Mouse+Liver+Sinusoidal+Endothelial+Cells/pmc12143466-32-0-10
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c57bl 6 mouse intestinal mesenteric primary endothelial cells  (AcceGen Biotechnology)
93
AcceGen Biotechnology c57bl 6 mouse intestinal mesenteric primary endothelial cells
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57bl 6 Mouse Intestinal Mesenteric Primary Endothelial Cells, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse%3A+c57bl/C57BL+6+Mouse+Intestinal+Mesenteric+Endothelial+Cells/bio_rxiv__2024__05__20__595057-36-0-7
Average 93 stars, based on 1 article reviews
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c57bl 6 mouse thymus  (AMS Biotechnology)
97
AMS Biotechnology c57bl 6 mouse thymus
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57bl 6 Mouse Thymus, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c57bl 6j background apoe  (Chem Impex International)
95
Chem Impex International c57bl 6j background apoe
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57bl 6j Background Apoe, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c57bl 6 mice  (AcceGen Biotechnology)
93
AcceGen Biotechnology c57bl 6 mice
Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 <t>MEFs</t> (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006
C57bl 6 Mice, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse%3A+c57bl/C57BL+6+Mouse+Primary+Tracheal+and+Bronchial+Epithelial+Cells/10__1016_slash_j__envint__2025__109772-67-7-9
Average 93 stars, based on 1 article reviews
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splenocytes  (iQ Biosciences)
94
iQ Biosciences splenocytes
NG-ISACs activate myeloid and lymphoid cells independent of ADCC and ADCP. A, FcγR-mediated activation was assessed using Jurkat-Lucia NFAT-CD16 and NFAT-CD32 reporter cells cocultured with CFPAC-1 tumor cells. ISAC-induced NFAT signaling was measured by Lucia luciferase activity 6 hours after incubation. Glycosylated ISACs are shown with dashed lines whereas NG-ISACs are shown with solid lines. B and C, Mouse BMDM-M2 ( B ) and <t>splenocytes</t> ( C ) were stimulated with E104 for 24 hours, followed by analysis of IL6 production using ELISA. D and E, RAW-Dual reporter macrophages were cocultured with BxPC-3‒TROP2 high ( D ) or CFPAC-1‒TROP2 medium tumor cells ( E ) in the presence of NG-ISACs or controls. Activation of NF-κB signaling pathways was measured. H, Mouse BMDM-M2 cells were cocultured with BxPC-3‒TROP2 high tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. I, Mouse splenocytes were cocultured with hTROP2-expressing tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. Data are presented as the mean ± SD ( n = 3), and EC 50 values are from three independent experiments. OD, optical density; RLU, relative light units.
Splenocytes, supplied by iQ Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatoma cell line  (ATCC)
86
ATCC mouse hepatoma cell line
NG-ISACs activate myeloid and lymphoid cells independent of ADCC and ADCP. A, FcγR-mediated activation was assessed using Jurkat-Lucia NFAT-CD16 and NFAT-CD32 reporter cells cocultured with CFPAC-1 tumor cells. ISAC-induced NFAT signaling was measured by Lucia luciferase activity 6 hours after incubation. Glycosylated ISACs are shown with dashed lines whereas NG-ISACs are shown with solid lines. B and C, Mouse BMDM-M2 ( B ) and <t>splenocytes</t> ( C ) were stimulated with E104 for 24 hours, followed by analysis of IL6 production using ELISA. D and E, RAW-Dual reporter macrophages were cocultured with BxPC-3‒TROP2 high ( D ) or CFPAC-1‒TROP2 medium tumor cells ( E ) in the presence of NG-ISACs or controls. Activation of NF-κB signaling pathways was measured. H, Mouse BMDM-M2 cells were cocultured with BxPC-3‒TROP2 high tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. I, Mouse splenocytes were cocultured with hTROP2-expressing tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. Data are presented as the mean ± SD ( n = 3), and EC 50 values are from three independent experiments. OD, optical density; RLU, relative light units.
Mouse Hepatoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 MEFs (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006

Journal: PLoS pathogens

Article Title: Virion assembly factories in the nucleus of polyomavirus-infected cells.

doi: 10.1371/journal.ppat.1002630

Figure Lengend Snippet: Figure 6. Tubular structures contain VP1. PyV-infected 3T3 cells or PML2/2 MEFs (MOI of 10–20 pfu/cell) at 32 hpi were frozen by high pressure and processed by cryo-substitution for immunoelectron microscopy. Thin sections (45 nm or 70 nm) of Lowicryl-embedded samples were stained with either anti-PML or anti-VP1 antibodies followed by a secondary antibody conjugated to 10 or 15 nm colloidal gold. Top panels: 45 nm sections stained for the PML protein; white arrows, anti-PML staining, black arrow, tubular structures. Bottom panels: 70 nm sections stained for VP1; white arrows, anti-VP1 staining, black arrows, tubular structures. doi:10.1371/journal.ppat.1002630.g006

Article Snippet: C57 mouse embryonic fibroblasts (MEF) were obtained from ATCC (SCRC-1008; Manassas, VA) and served as a wild-type MEF control.

Techniques: Infection, Immuno-Electron Microscopy, Staining

Figure 7. PyV DNA and T-antigen localization in PyV-infected PML2/2 MEFs. PML2/2 MEFs were infected with PyV at an MOI of 30–40 pfu/ cell. At 22 or 24 hpi cells were fixed, permeabilized, and co-stained with either anti-Tag and/or anti-MRE11a antibodies followed by AlexaFluor- conjugated secondary antibodies, a fluorescently-labeled PyV DNA FISH probe, and DAPI staining of nuclei. A) FISH for PyV DNA at 24 hpi followed by antibody staining for Tag. B) Infected cells were stained by FISH for PyV DNA at 22 hpi followed by antibody staining for MRE11 or co-stained for MRE11 and Tag. All images represent a 0.1 mm z-stack slice. doi:10.1371/journal.ppat.1002630.g007

Journal: PLoS pathogens

Article Title: Virion assembly factories in the nucleus of polyomavirus-infected cells.

doi: 10.1371/journal.ppat.1002630

Figure Lengend Snippet: Figure 7. PyV DNA and T-antigen localization in PyV-infected PML2/2 MEFs. PML2/2 MEFs were infected with PyV at an MOI of 30–40 pfu/ cell. At 22 or 24 hpi cells were fixed, permeabilized, and co-stained with either anti-Tag and/or anti-MRE11a antibodies followed by AlexaFluor- conjugated secondary antibodies, a fluorescently-labeled PyV DNA FISH probe, and DAPI staining of nuclei. A) FISH for PyV DNA at 24 hpi followed by antibody staining for Tag. B) Infected cells were stained by FISH for PyV DNA at 22 hpi followed by antibody staining for MRE11 or co-stained for MRE11 and Tag. All images represent a 0.1 mm z-stack slice. doi:10.1371/journal.ppat.1002630.g007

Article Snippet: C57 mouse embryonic fibroblasts (MEF) were obtained from ATCC (SCRC-1008; Manassas, VA) and served as a wild-type MEF control.

Techniques: Infection, Staining, Labeling

NG-ISACs activate myeloid and lymphoid cells independent of ADCC and ADCP. A, FcγR-mediated activation was assessed using Jurkat-Lucia NFAT-CD16 and NFAT-CD32 reporter cells cocultured with CFPAC-1 tumor cells. ISAC-induced NFAT signaling was measured by Lucia luciferase activity 6 hours after incubation. Glycosylated ISACs are shown with dashed lines whereas NG-ISACs are shown with solid lines. B and C, Mouse BMDM-M2 ( B ) and splenocytes ( C ) were stimulated with E104 for 24 hours, followed by analysis of IL6 production using ELISA. D and E, RAW-Dual reporter macrophages were cocultured with BxPC-3‒TROP2 high ( D ) or CFPAC-1‒TROP2 medium tumor cells ( E ) in the presence of NG-ISACs or controls. Activation of NF-κB signaling pathways was measured. H, Mouse BMDM-M2 cells were cocultured with BxPC-3‒TROP2 high tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. I, Mouse splenocytes were cocultured with hTROP2-expressing tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. Data are presented as the mean ± SD ( n = 3), and EC 50 values are from three independent experiments. OD, optical density; RLU, relative light units.

Journal: Molecular Cancer Therapeutics

Article Title: Nonglycosylated, Legumain-Cleavable ISACs Drive Potent Antitumor Immunotherapy via a Bystander Effect

doi: 10.1158/1535-7163.MCT-25-1153

Figure Lengend Snippet: NG-ISACs activate myeloid and lymphoid cells independent of ADCC and ADCP. A, FcγR-mediated activation was assessed using Jurkat-Lucia NFAT-CD16 and NFAT-CD32 reporter cells cocultured with CFPAC-1 tumor cells. ISAC-induced NFAT signaling was measured by Lucia luciferase activity 6 hours after incubation. Glycosylated ISACs are shown with dashed lines whereas NG-ISACs are shown with solid lines. B and C, Mouse BMDM-M2 ( B ) and splenocytes ( C ) were stimulated with E104 for 24 hours, followed by analysis of IL6 production using ELISA. D and E, RAW-Dual reporter macrophages were cocultured with BxPC-3‒TROP2 high ( D ) or CFPAC-1‒TROP2 medium tumor cells ( E ) in the presence of NG-ISACs or controls. Activation of NF-κB signaling pathways was measured. H, Mouse BMDM-M2 cells were cocultured with BxPC-3‒TROP2 high tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. I, Mouse splenocytes were cocultured with hTROP2-expressing tumor cells and stimulated with NG-ISACs for 24 hours. IL6 production was quantified by ELISA. Data are presented as the mean ± SD ( n = 3), and EC 50 values are from three independent experiments. OD, optical density; RLU, relative light units.

Article Snippet: Ramos-Blue reporter cells (2024, InvivoGen, cat. #rms-sp, RRID: CVCL_X591), human peripheral blood mononuclear cells (PBMC; male; iXCells Biotechnologies, November of 2024), mouse BMDM-M2 and splenocytes (March 2024; iQ Biosciences; female, IQB-MSP202), BxPC-3 cells (2023, ATCC, cat. #CRL-1687, RRID: CVCL_0186), RAW-Dual reporter cells (May 2019, InvivoGen, cat. #rawd-ismip, RRID: CVCL_A7ZK), MC38-hTROP2 (April 2024, Kyinno Biotechnology, cat. #KC-1698), Jurkat-Lucia NFAT-CD16 (June 2024, InvivoGen, cat. #jkt-nfat-cd16, RRID: CVCL_A7ZT), and NFAT-CD32 (August 2024, InvivoGen, cat. #jkt-nfat-cd32, RRID: CVCL_A7ZU) were also obtained.

Techniques: Activation Assay, Luciferase, Activity Assay, Incubation, Enzyme-linked Immunosorbent Assay, Protein-Protein interactions, Expressing